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Association of CHRNA5 total mRNA expression with variants within and flanking the CHRNA5 gene.

Journal: PLoS ONE

Article Title: Cis -Regulatory Variants Affect CHRNA5 mRNA Expression in Populations of African and European Ancestry

doi: 10.1371/journal.pone.0080204

Figure Lengend Snippet: Association of CHRNA5 total mRNA expression with variants within and flanking the CHRNA5 gene.

Article Snippet: TaqMan genotyping assays for rs16969968 (Life technologies, C_26000428_20) and rs615470 (Life technologies, C_18757_10) are within the CHRNA5 transcript, and were used for allele specific quantitative RT-PCR in each genomic DNA and cDNA.

Techniques: Expressing

Minor allele of rs880395 (A allele), rs3841324 (S allele) and rs588765 (T allele) are associated with increased total mRNA expression of CHRNA5 in human frontal cortex of African Americans.

Journal: PLoS ONE

Article Title: Cis -Regulatory Variants Affect CHRNA5 mRNA Expression in Populations of African and European Ancestry

doi: 10.1371/journal.pone.0080204

Figure Lengend Snippet: Minor allele of rs880395 (A allele), rs3841324 (S allele) and rs588765 (T allele) are associated with increased total mRNA expression of CHRNA5 in human frontal cortex of African Americans.

Article Snippet: TaqMan genotyping assays for rs16969968 (Life technologies, C_26000428_20) and rs615470 (Life technologies, C_18757_10) are within the CHRNA5 transcript, and were used for allele specific quantitative RT-PCR in each genomic DNA and cDNA.

Techniques: Expressing

Summary of strengths, limitations and available tools of the current PDB strategies.

Journal: Frontiers in Molecular Biosciences

Article Title: Proximity-dependent biotinylation technologies for mapping RNA-protein interactions in live cells

doi: 10.3389/fmolb.2022.1062448

Figure Lengend Snippet: Summary of strengths, limitations and available tools of the current PDB strategies.

Article Snippet: AVAILABLE TOOLS , • Plasmid to clone BoxB‐tagged RNAs (Addgene #107253) , , • Plasmid encoding for MCP-APEX2 (Addgene #154936) , , • Plasmid encoding for CARPID BASU-dCasRx (Addgene #153209), CARPID dCasRx-BASU (Addgene #153303), dCas13d-dsRBD-APEX2 (Addgene #154939) , • Addgene plasmids encoding for APEX2-OMM (#79056), ERM-APEX2 (#79055), mito-APEX2 (#72480), APEX2-SENP (#129276), APEX2-eIF41 (#129645), APEX2-eIFE1 (#129644), C1-APEX2 (#129641), APEX2-FBL (#187577), APEX2-SRSF7 (#187582), APEX2-SRSF1 (#187575), APEX2-PML (#187583), APEX2-SP100 (#187584), APEX2-NPAT (#187585), APEX2-LMNA (#187576).

Techniques: CRISPR, Purification, Binding Assay, Plasmid Preparation, Expressing, Positive Control